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KMID : 0364820150510020181
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Korean Journal of Microbiology
2015 Volume.51 No. 2 p.181 ~ p.185
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Effects of Tho2, a component of THO complex, on growth and mRNA export in fission yeast
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Koh Eun-Jin
Yoon Jin-Ho
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Abstract
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Tho2/THOC2 is a subunit of the THO complex that plays importantr roles in mRNP biogenesis connecting transcription with mRNA maturation and export. A fission yeast, Schizosaccharomyces pombe, ortholog of Tho2/THOC2 was identified from the genome database. Tetrad analysis showd that the S. pombe tho2 is essential for growth. Repression or overexpression of the tho2 gene caused growth defect with elongated cells, abnormal DNA distribution, and accumulation of poly(A)+ RNA in the nucleus. And the functional GFP-Tho2 protein is localized mainly in the nucleus. Yeast two-hybrid analysis showed that Tho2 interacted with Tex1, another subunit of THO complex. These results suggest that S. pombe Tho2 is also involved in mRNA export from the nucleus and is a component of THO complex.
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KEYWORD
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S. pombe, mRNA export, THO complex, Tho2/Thoc2
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